Episode 95: Despite using a powerful healing technique, Kabuto’s wounds are too extensive for him to deal with. When Naruto collapses due to the severity of Kabuto’s last attack, Tsunade does what she can, determined to save him. Her efforts work, and he regains consciousness long enough to claim the necklace he has won. Orochimaru, recognizing Naruto as a potential future threat, tries to kill him. Tsunade, determined to make sure Naruto becomes Hokage, overcomes her fear of blood and uses herself as a human shield to block the attack. When asked why she would risk her life to save him, Tsunade replies that it is her duty as the Fifth Hokage, finally accepting the role she was offered. Intending to eliminate his former teammates, Orochimaru summons Manda while Tsunade and Jiraiya summon Katsuyu and Gamabunta respectively.
Episode 96: Katsuyu and Gamabunta double-team Manda, though the latter is able to hold his own. Using her inhuman strength, Tsunade is able to pin Manda down and delivers a finishing blow to Orochimaru. Deciding to use his last resort to regain the use of his arms, Orochimaru retreats with Kabuto, leaving Tsunade, Jiraiya, Shizune, and Naruto to their own devices. Once Naruto recovers and learns of Tsunade’s acceptance of the Hokage title, he remarks that she is an undeserving of the job due to the fact that the Third was better in every possible way. The two step outside to have another fight, though this time Tsunade gives Naruto a kiss on the forehead instead of a blow to the face. The group then sets off for Konoha.